Articles on SPEPS
21.Earth, Isotopes and Organics
This special issue highlights the latest scientific findings in the issues and key words of “Earth, Isotopes and Organics.” The Earth harbors the diverse elements that make up organic molecules with Carbon (C), Hydrogen (H), Nitrogen (N), Oxygen (O), Sulfur (S), and Phosphorus (P). Major inorganic elements represented by magnesium (Mg) and iron (Fe) also have important roles for biosphere. Those diverse elements have also stable and radioactive isotopes in their natural compositions. Standardization through international standard references is underway to develop highly accurate and precise analytical methodology. These highly precise isotope measurements are robust chemical tools in deciphering the interaction between biotic and abiotic factors. Therefore, this special issue can be defined as the understanding of the life science and chemistry on the Earth (e.g., biosphere, hydrosphere, and geosphere) through the representation of essential elements associated with inorganic and organic molecules in nature. In this special issues, further perspectives for state-of-the-art developments in compound-specific isotope analysis will be overviewed for next decadal progresses.
Edited by: Yoshinori Takano, Yoshito Chikaraishi, Thomas Blattmann, Mark Lever, Kensei Kobayashi, Yusuke Okazaki
Methodologies articles: Interdisciplinary research
Published: December 1, 2025
Purification of hydroxyproline for small-scale radiocarbon dating verified using archaeological bone collagen
Yu Itahashi, Yoshinori Takano, Yoshito Chikaraishi, Nanako O. Ogawa, Naoto F. Ishikawa, Naohiko Ohkouchi, Masako Yamane, Yusuke Yokoyama, Minoru Yoneda
Research articles: Biogeosciences
Published: October 30, 2025
Application of Microsublimation for Sample Purification in Compound-Specific Radiocarbon Analysis
Christian Heusser, Caroline Welte, Lukas Wacker, Kai Sebastian Nakajima, Thomas Blattmann, Negar Haghipour, Timothy Ian Eglinton
Methodologies articles: Biogeosciences
Published: October 29, 2025
Nanomolar-scale Sulfate Sulfur Isotope Analysis Using a Combined Ion Chromatography-Barite Precipitation and nano-EA/IRMS
Nanako O OGAWA, Toshihiro Yoshimura, Takeru Kochi, Junichiro Kuroda, Yoshinori Takano, Naohiko Ohkouchi
Methodologies articles: Interdisciplinary research
Published: October 29, 2025
Challenges in extraction and quantification of cellulose from deep-sea sediments
Noriyuki Isobe, Sachiko Kawada, Yuto Kaku, Kiyohiko Igarashi
Research articles: Biogeosciences
Published: October 16, 2025
Fatty Acids as the Main Energy Source in Polyps of the Jellyfish (Cnidaria: Aurelia aurita) for Survival Strategy under Overpopulation: Insights from Compound-Specific Isotope Analysis
Nayeon Park, Hyuntae Choi, Yuko Takizawa,Yoshito Chikaraishi
Research articles: Biogeosciences
Published: October 15, 2025
Nitrogen isotopic composition of amino acids reveals a variety of trophic ecology of organisms inhabiting chemosynthesis-based ecosystems
Masashi Tsuchiya, Takao Yoshida, Yoshito Chikaraishi, Nanako O Ogawa, Hidetaka Nomaki, Yoshihiro Fujiwara, Yasuhiko T Yamaguchi, Yohei Tada, Robert G Jenkins, Yoko Sasaki, Katsunori Fujikura, Naohiko Ohkouchi
Research articles: Biogeosciences
Published: October 14, 2025
Elucidating the isotopic dynamics of nitrate and ammonium in the Fuji River using a Lagrangian approach: stable nitrate and fractionating ammonium
Công Thuận Nguyễn, Tomoya Iwata, Yuta Ikeda, Midori Yano, Akiko Makabe, Keisuke Koba
Research articles: Biogeosciences
Published: October 1, 2025
Methane- and hydrogen-dependent prokaryotic deep biosphere at the Suwa Basin, Japan: impacts of hydrogeological processes on subsurface prokaryotic ecology at the boundary between the North American and the Eurasian Plates
Hiroki Nishimura, Atsushi Urai, Yohei Matsui, Toshihiro Yoshimura, Miyuki Ogawara, Hiroyuki Imachi, Yosuke Miyairi, Yusuke Yokoyama, Yuichi Miyabara, Yoshio Takahashi, Yoshinori Takano
Research articles: Biogeosciences
Published: September 30, 2025
Fractionation of nitrogen isotopes in the enzymatic deamination of alanine: an insight into the quantitative evaluation of anaerobic metabolism in ecosystems
Yuko Takizawa, Hyuntae Choi, Mio Shibuya, Nayeon Park, Prarthana S. Dharampal, Shawn A. Steffan, Yoshito Chikaraishi
Research articles: Biogeosciences
Published: September 30, 2025
A compound-specific method for measuring the stable carbon isotopic composition of amino acids: elimination of the effect of isotopic fractionation during derivatization
Yoshito Chikaraishi, Hyuntae Choi, Nayeon Park,Yuko Takizawa
Research articles: Biogeosciences
Published: September 29, 2025
Reconstructing the body temperature of extinct marine reptiles
Takashi Sambuichi, Urumu Tsunogai, Fumiko Nakagawa
Methodologies articles: Biogeosciences
Published: September 29, 2025
Chromatographic isolation for performing enantiomer-specific radiocarbon analysis (ESRA): first method development for individual L- and D-amino acids
Thomas M. Blattmann, Yuchen Sun, Nanako O. Ogawa, Yoshinori Takano, Negar Haghipour, Naoto F. Ishikawa, Timothy I. Eglinton, Naohiko Ohkouchi
Research articles: Biogeosciences
Published: August 1, 2025
Gleaning of δ15N in ecosystems and its animal-organ isotope data: perspectives for the construction of the isotopically ordered world
Eitaro Wada, Yoshihisa Kudo, Hiroshi Mizutani, Hiroshi Minami, Kenji Matsubara, Yoshihiro Yamada, Nanako O. Ogawa, Yukihiro Kohmatsu, Naoko Nishizawa, Isamu Shimizu, Reiichiro Ishii, Fujio Hyodo, Maki N. Aita, Wataru Sakamoto, Naohiko Ohkouchi
Research articles: Biogeosciences
Published: July 23, 2025
Nitrogen and carbon isotopic relationships in the diet and eye lenses of chub mackerel revealed in a laboratory rearing experiment
Chisato Yoshikawa, Nanako O. Ogawa, Naoto F. Ishikawa, Michio Yoneda, Ryuji Yukami, Shin-ichi Ito, Naohiko Ohkouchi
Research articles: Biogeosciences
Published: June 4, 2025
Carnivorous plants: unveiling trophic identity and advanced nitrogen acquisition strategies
Rong Fan, Yuko Takizawa, Prarthana S. Dharampal, Shawn A. Steffan and Yoshito Chikaraishi
Research articles: Biogeosciences
Published: April 16, 2025
Evidence of methanogenic activity associated with past fish farming in Lake Soyang, South Korea
Dong-Hun Lee, Jung-Hyun Kim, Sujin Kang, Dahae Kim, Kyung-Hoon Shin
Research articles: Biogeosciences
Published: March 6, 2025
Magnesium isotopic variation in marine fish organs
Toshihiro Yoshimura, Naoto F. Ishikawa, Nanako O. Ogawa, Soichiro Kusaka, Shigeyuki Wakaki, Tsuyoshi Ishikawa, Kazuya Nagaishi, Masanori Kaneko, Yuta Isaji, Yoshito Chikaraishi, Naohiko Ohkouchi
Research articles: Biogeosciences
Published: February 11, 2025
Distributions of heme B and its implications on Fe biogeochemistry in the eastern Indian Ocean and the western North Pacific Ocean
Yuta Isaji, Koji Sugie, Naoto F. Ishikawa, Nanako O. Ogawa, Kazuhiko Matsumoto, Chisato Yoshikawa, Hajime Obata, Makio C. Honda, Naohiko Ohkouchi
Research articles: Biogeosciences
Published: February 10, 2025
Highly effective energy transfer in the autotroph-heterotroph symbiosis: insights from compound-specific isotope analysis of amino acids
Jincen Li, Mio Shibuya, Yuko Takizawa, Yoshito Chikaraishi
Research articles: Biogeosciences
Published: February 5, 2025
A large elevation in 15N/14N of collagenous amino acids: an insight from starvation experiments of marine organisms
Hyuntae Choi, Yuko Takizawa, Nayeon Park, Yoshito Chikaraishi
Research articles: Biogeosciences
Published: January 29, 2025
Isotope analysis of birds’ eye lens provides early-life information
Emi A. Hasegawa, Jun Matsubayashi, Ichiro Tayasu, Tatsuhiko Goto, Haruka Inoue, Axel G. Rossberg, Chikage Yoshimizu, Masaru Hasegawa, Takumi Akasaka
Research articles: Biogeosciences
Published: January 15, 2025
Differential use of multiple food sources at a bathyal benthic ecosystem in the central Sagami Bay revealed by amino acid nitrogen isotopic compositions
Hidetaka Nomaki, Yoshito Chikaraishi, Nanako O. Ogawa, Koh Maki, Motohiro Shimanaga, Hisami Suga, Masashi Tsuchiya, Toshi Nagata, Naohiko Ohkouchi
Research articles: Biogeosciences
Published: January 3, 2025
Spatiotemporal variations in integrated trophic positions of stream macroinvertebrate communities
Naoto F. Ishikawa, Yoshitoshi Uehara, Takuya Ishida, Tohru Ikeya, Satoshi Asano, Chia-Ying Ko, Tomoya Iwata, Ichiro Tayasu, Naohiko Ohkouchi, Noboru Okuda
Research articles: Biogeosciences
Published: October 2, 2024
Dietary partitioning in sympatric Paradoxurinae civets in Borneo suggested by compound-specific nitrogen isotope analysis of amino acids
Miyabi Nakabayashi, Takumi Tsutaya, Abdul Hamid Ahmad, Yoko Sasaki, Nanako O. Ogawa, Naoto F. Ishikawa, Naohiko Ohkouchi
Research articles: Biogeosciences
Published: September 12, 2024
Natural 14C abundances and stable isotopes suggest discrete uptake routes for carbon and nitrogen in cold seep animals
Hidetaka Nomaki, Shigeaki Kojima, Yosuke Miyairi, Yusuke Yokoyama, Chong Chen
Research articles: Biogeosciences
Published: August 22, 2024
Regulation of nitrogen isotopic ratios of cellular components
Naohiko Ohkouchi, Yuta Isaji, Nanako O. Ogawa